In line with Martin and colleagues [29] we found that women more often engaged in walking, swimming and dancing compared to men, while men more often performed jogging, cycling and winter sports. Our data also showed that men performed a higher amount of sessions with domestic activities and combined endurance and resistance training compared to women. The sex differences were the same in both training groups, indicating that disparities in type of exercise between older women and men are independent of the exercise intensity they are instructed to perform.
Check your company's stock plan for the allowed methods and procedures. Once you do give notice to exercise with payment as required under your stock plan, it is unlikely that you can then change your decision to exercise or the exercise method, at least for tax purposes. This is illustrated by the case of Walter v. Commissioner, decided by the US Tax Court (TC Memo 2007-2, January 3, 2007). The employee attempted to change his original exercise instructions from a same-day sale to a cash exercise/hold. The Tax Court did not let him, holding that he had beneficial ownership of the stock when he initially exercised the options following the conditions under his stock plan.
The goal with exercise is to work WITH our bodies and slowly condition over time. This is not a quick process because creating a “heal-thy” lifestyle takes diligence and consistency. The best way to avoid Post Exercise Malaise is to increase both duration and intensity SLOWLY over time and include adequate rest breaks and recovery time in between workouts.
Exercising stock options should not be a passive event that happens after a given amount of time. It's rather like playing a hand of cards: if your plays are strategic, you'll probably "know when to hold them and when to fold them." Many alternatives and trade-offs need to be considered. But, as always, rules, requirements, and regulations govern this area, and this is where we begin.
Preliminary evidence from a 2012 review indicated that physical training for up to four months may increase sleep quality in adults over 40 years of age.[78] A 2010 review suggested that exercise generally improved sleep for most people, and may help with insomnia, but there is insufficient evidence to draw detailed conclusions about the relationship between exercise and sleep.[79]
Take the stairs. You know, some people pay to use stairs (read: they buy a stair stepper machine), so if you work or live above the first floor, consider using your stairs as a privilege. Using the stairs is great cardiovascular exercise, and also tones your calves, hamstrings, glutes, quadriceps, and abs. Next time you're about to take the elevator or escalator, take the stairs instead. If you have some time to spare, take an extra trip up and down. Or, try a stair-based workout like this one.
Mix-and-match interval training works magic in Natalie Jill's Rev4 Rev It Up. The four 10-minute routines hit different trouble zones so you can do them as stand-alones—"I definitely felt I got a good workout after each," one tester said—or combine them for a total-body session. Testers loved that they could "switch things up for time-pressed mornings" and gave props to instructor Jill's "nice energy." Expect a variety of planks and booty-shaping moves.
PiYo isn't like standard Pilates and yoga classes that make you hold long, intense poses, or lead you through dozens of repetitive, microscopic core movements. PiYo speeds everything up—including your results—by introducing you to dynamic, flowing sequences that can burn serious calories at the same time as they lengthen and tone your muscles and increase your flexibility.
Various weighted dumbbells. Some exercises require heavier weights, while others will need lighter weights or none at all. Try to have a range of dumbbells: a light set (3 to 5 pounds for women, 5 to 8 pounds for men), a medium set (5 to 10 pounds for women, 10 to 15 pounds for men), and a heavy set (10 to 20 pounds for women, 15 to 30 pounds for men).

Multiple new, yet generally typical MMB methods originated after 1950, including the Feldenkrais Method;51 Reese M [Internet]. A biography of Moshe Feldenkrais. 2015 Aug 30 [cited 2015 Aug 30]. Available from: http://www.feldenkrais.com/moshe-feldenkrais. [Google Scholar] the modern form of Calisthenics;52 Ozer Kaya D, Duzgun I, Baltaci G, Karacan S, Colakoglu F. Effects of calisthenics and Pilates exercises on coordination and proprioception in adult women: a randomized controlled trial. J Sport Rehabil. 2012;21(3):235–43. [Google Scholar] Gyrokinesis and Gyrotonic by Horvath;53 Gyrotonic and Gyrokinesis [Internet]. 2015 Aug 30 [cited 2015 Aug 30]. Available from: https://www.gyrotonic.com. [Google Scholar] Gaga by Naharin54 Gaga [Internet]. 2015 Aug 28 [cited 2015 Aug 30]. Available from: http://gagapeople.com/english/about-gaga/. [Google Scholar] and CoreAlign55 The CoreAlign [Internet]. 2015 Aug 30 [cited 2015 Aug 30]. Available from: http://www.pilates.com/BBAPP/V/education/corealign/index.html. [Google Scholar] and Human Harmony by Hoffman.56 Human Harmony Training [Internet]. 2015 Aug 30 [cited 2015 Aug 30]. Available from: http://www.fffforlife.com/#!human-harmony-training/c66t. [Google Scholar] In recent decades, many allied health professionals and therapists have embraced the MMB concepts as an integral part of their practice and detailed in mainstream scientific literature their research findings on its effectiveness.57–59 Wells C, Kolt GS, Marshall P, Hill B, Bialocerkowski A. Effectiveness of Pilates exercise in treating people with chronic low back pain: a systematic review of systematic reviews. BMC Med Res Methodol. 2013;13:7. Available from: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3563510/10.1186/1471-2288-13-7
In the realm of fitness, three-month workout programs dominate the landscape. You’ve even seen plenty of them in our magazine over the years. Are they effective? Absolutely. But we’re going to let you in on an interesting secret: It doesn’t necessarily take 8 or 12 weeks to get your feet wet in the gym. Not that you’ll be a seasoned vet after four weeks, but if you can just get that first month under your belt, you’ll get yourself over the proverbial hump, where so many fail and give up, and set the stage for a lifetime of muscle gains.
Exercise is a key part of staying healthy, but figuring out how to get more active can be tough. If you’re not used to physical activity, start slow. Go for 10 to 15 minute walks, and work your way up to briskly walking or jogging for 30 minutes daily. Try adding strengthening exercises 2 or 3 days per week, and consider boosting your flexibility with yoga or Pilates classes. Whenever you work out, always listen to your body’s limits, and ask your doctor for advice if you have a history of any medical issues.

Lose yourself in the high-energy rhythm of the Pound Rockout Results System, a five-disc sweatfest in which you wield drumsticks (aka Ripstix) instead of weights. "The drumming takes your mind off your muscles hurting!" one tester marveled. You'll "constantly tap the sticks" in each routine—core, upper body, lower body, intervals, tune-up and jam session—for a "totally unique" cardio blast.

One of the easiest parts of starting an exercise program is deciding to do it. Usually there's something inspiring you to make a change: Maybe you tried on a pair of jeans that were too tight or there's an upcoming event—a reunion, wedding, or party—where you're going to see people you haven't seen in a while. Whatever it is, you're motivated, you're excited, and the fantasy of a new, slimmer you is enough to inspire you.
In 1937, Randell’s number-two Thomas was sent to Australia to lecture on the St Thomas Project, teach the exercises, and assist local physiotherapists with the program’s implementation.35 Evans EP. The history of the New South Wales branch of the Australian Physiotherapy Association. Aust J Physiother. 1955;1(2):76–8.10.1016/S0004-9514(14)60817-5[Crossref] [Google Scholar] There are multiple indications of an unknown video Thomas made during this visit, and it was reported later that as a result of the visit, the St Thomas method was implemented in maternity hospitals in Sydney and Melbourne; the exercises were used by physiotherapists on an individual basis and that Thomas was remembered as a pioneer of Australian antenatal physiotherapy.29,35,36 Sydney Morning Herald [Internet]. Thomas BM: Obituary (1940). 2014 Oct 23 [cited 2015 Aug 30]. Available from: http://www.tiveyfamilytree.com/Barbara-Mortimer-Thomas-Death-Article-SMH-11-9-1940.htm.

This is an extremely high-skill movement, and is one of two Olympic Weightlifting events. HOW TO DO IT: Start with the bar on the ground with your feet hip-width apart. With your hands wide on the bar, keep a big chest as you deadlift the weight off the ground (similar to the beginning of the clean). Pull from the floor with your arms in a locked position. Then, drive your hips and pull the bar as high as possible. As you receive the bar overhead, drop down as quickly as possible and lock your arms into place in a squat position with the bar overhead. MUSCLES USED: Glutes, quads, hamstrings, calves, traps, core, shoulders and back.


After performing the two exercises of the Alfredson protocol, you may feel soreness or pain in the back of your ankle by your Achilles' tendon and soreness in your calf muscles.  This soreness will last for a day, and the soreness will become much less as you progress with the exercises over the course of weeks.  The Alfredson protocol indicates that you continue with the exercises unless the pain becomes disabling.  If this occurs, consult your doctor.
Exercise and physical activity fall into four basic categories—endurance, strength, balance, and flexibility. Most people tend to focus on one activity or type of exercise and think they’re doing enough. Each type is different, though. Doing them all will give you more benefits. Mixing it up also helps to reduce boredom and cut your risk of injury.
Evidence from HIIT studies conducted under controlled laboratory conditions has provided proof-of-concept of efficacy [9]. However, it has been argued that HIIT has high efficacy but low effectiveness [16], and long-term exercise interventions carried out under free-living conditions have been asked for to investigate whether HIIT is feasible as a public health initiative among older adults [9, 16]. Our data showed that both training groups reported on average more than two exercise sessions per week throughout the year. Approximately 60% of the sessions in the HIIT group were performed with a self-reported high-intensity (≥15 Borg scale), indicating that older adults are able to perform HIIT over a long time-period without strict supervision. However, women had a lower proportion of sessions with high-intensity exercise compared to men. This result is in line with previous findings that women (aged 60–67 years) are less likely than men to prefer vigorous physical activity [23].
The aims of the present study were to assess the reliability of a novel high intensity OLDE protocol to measure muscle endurance, and to describe the isokinetic muscle fatigue induced by high intensity OLDE and its recovery. We demonstrated that our novel high intensity OLDE protocol can be used as a reliable measure of muscle endurance, and that isokinetic muscle fatigue recovers and plateaus within ~ 30 s following exhaustion. Therefore, the novel high intensity OLDE protocol tested in this study might provide an interesting tool to investigate muscle fatigue and muscle endurance.
Association of exercise type with sex in the MCT (a) and HIIT (b) groups. Data are presented as proportions of the total number of exercise sessions. Other type of endurance; treadmill, cross trainer, aerobics etc., Domestic activities; housework, gardening etc., Other: golf, bowling, horseback riding etc. *Significantly different from men (p < 0.05)
During your workout you have “target” heart rate zones that are expressed as a percentage of your max heart rate. For low-intensity cardio, you want to aim for 60 to 70 percent of your max heart rate, for moderate-intensity cardio the goal is 70 to 85 percent, and for high-intensity cardio, 85 percent or above. This can help you see if you’re really working as hard as you think you are and adjust as needed to make sure you’re hitting your workout goals. Here’s how to calculate your max and target heart rate zones.
After 5 min warm up at 20% of peak power output, subjects performed a time to exhaustion at 85% of peak power output. Exhaustion was defined as a decrease in cadence below 40 cpm for a duration ≥ 10 s or when the subject voluntarily stopped. Subjects were not aware of the time elapsed during the time to exhaustion test. Verbal encouragements were provided by an experimenter naïve of time to exhaustion during the previous sessions.

Rest-Pause Sets. The body is an amazing machine, with the right amount of rest it can surprise us with its tenacity. With a weight near your 3 or 5 rep maximum, perform as many reps as possible, then re-rack. Rest for 10 to 15 seconds, then grab it again and go at it. Make sure to keep correct form, and go/rest until you can't budge the bar. Make sure to only do this once.
In the third week of the program we step it up to a three-day training split: Train all “pushing” bodyparts (chest, shoulders, triceps) on Day 1; hit the “pulling” bodyparts (back, biceps) and abs on Day 2; and work your lower body (quads, glutes, hamstrings, calves) on Day 3. As in Week 2, you train each bodypart twice a week, so you’ll hit the gym six days this week.
In 1912, John Shields Fairbairn, a leading consultant obstetrician at St Thomas Maternity Hospital, London, started a program to revolutionize the medical approach to child delivery.22 Polden M, Mantle J. Physiotherapy in obstetrics and gynaecology. 2nd ed. Oxford: Butterworth Heinemann; 2004. [Google Scholar] This aimed to replace the 19th century medical practice of heavily medicating women during labor and the common use of force to deliver. To implement his vision of providing pregnant women with education and natural physical health for childbirth and recovery, Fairbairn chose Midwife and Physiotherapist Minnie Randell (1875–1974) to lead the newly founded St Thomas School of Physiotherapy, which served as the project’s education and training center.22,23 Polden M, Mantle J. Physiotherapy in obstetrics and gynaecology. 2nd ed. Oxford: Butterworth Heinemann; 2004.
* Exercise isn't supposed to be fun or enjoyable (that's what recreation is for). Exercise is a means-to-an-end. It shouldn't be something you look forward to, nor should you dread doing it; it should just be part of your lifestyle... like eating or sleeping. If you DO look forward to it, and even crave it, you could be hooked on the endorphin rush you get from doing an activity that is considered by the body to be over-doing it. And since the body would rather you not do this, it would be prudent to respect the body's wishes.
Wellcome Library [Internet]. Rodway H. Training for childbirth - and after (1940). 2015 Sep 24 [cited 2015 Oct 3]. Available from: http://wellcomelibrary.org/player/b16729006#?asi=0&ai=0.  Morris,33 Morris M. Basic physical training. London: Heinemann; 1937. [Google Scholar] and Vaughan,47 Youtube [Internet]. Vaughan, K. Childbirth as an athletic feat (1939). 2009 Feb 23 [cited 2015 Aug 30]. Available from: https://www.youtube.com/watch?v=g9wRBWDxReY. [Google Scholar] Pilates emphasized breathing techniques, training in sunshine and fresh air. Pilates also advocated the use of minimal and light-fitting clothing, recommended cold exposure and the importance of bathing and treating the skin.45,46 Pilates J. Your health. Nevada: Presentation Dynamics; 1934.
Two incremental graded exercise tests until exhaustion were performed, with 4 h of rest in between. One hour before each test, the athletes received a standardised meal (2315 kJ, 73% carbohydrate, 19% protein, 8% fat). Athletes arrived in the laboratory at 07:00 after an overnight fast. The first blood sample was collected as they arrived. Immediately after the first exercise test, the second blood sample was drawn. The third and fourth blood samples were drawn before and immediately after the second test. A schematic overview of the protocol can be found in fig 1. Because it is known that venepuncture increases blood prolactin, going back to baseline within 30 min, blood was drawn before and after each test (four punctures) creating the same “stress” in each situation. The study protocol was approved by the university ethical committee.
This DVD is focused on strength training — you can choose whether you want to do an upper body workout, a lower body workout, an abs and back routine, or a quickie 10-minute total body workout. But don't think you have to already be super buff to jump in: This workout is designed for people of any fitness level — though you will need some equipment for it, like a stretch band and exercise ball.

Warm up. This is the act of preparing your body for the stress of exercise. The body can be warmed up with light intensity aerobic movements like walking slowly. These movements increase blood flow, which in turn heats up muscles and joints. "Think of it as a lube job for the body," Bryant explains. At the end of your warm-up, it's a good idea to do a little light stretching.
Before beginning any workout program, it’s always a good idea to consult your physician. Individuals with pre-existing injuries or medical conditions or those who don’t have an accurate knowledge of their current physical fitness level should always begin cautiously. It’s also extremely important to perform all workouts, no matter what fitness method you’re doing, under the supervision of a certified instructor. Their guidance and knowledge of proper form and technique will help you to achieve maximum results while avoiding injuries. They can also help you to safely modify workout activities to match your current fitness levels and goals. Whether you attend classes in a studio or at home through DVDs or streamed videos, the presence of a trained instructor is imperative.
"CrossFit differentiates itself by being constantly varied in both movements and time domains," Mandelbaum says. "You might have a day in the box with a four-minute sprint workout one day, and then come in the next day for a 15-minute moderate-to-fast-paced workout featuring three movements that need to be repeated in a cycle or round until the time clock runs out."

For Paced Audio Workouts: MotionTraxx (iPhone or iPad) - This music-based app is another great option for home exercisers, particularly if you're a walker or runner. Deekron the Fitness DJ has put together an incredible variety of music mixes, all set at different beats per minute, so you can find the perfect pace for any workout - Walking, running, lifting weights or other activities. There are also coached workouts (these cost extra) available at iTunes.
Are you new to the gym and not sure where to start? Looking to add more variety into your workout? Or are you wanting to master a move to perfection? Then look no further. Learn how to perform exercise moves safely and effectively with our exercise database, which includes top tips from our qualified trainers, video demonstrations, and a step by step guide.
The Push Press is a move that incorporates your entire body. While the strict press focuses only on the upper body, the push press incorporates the lower body to drive the bar up overhead. This synchronic movement is great for building power and pure strength. HOW TO DO IT: Start with the bar across your shoulders. Your hands position on the bar should be just slightly outside of your shoulders, and your feet should be shoulder-width apart. Brace your core, dip slightly into a quarter squat and squeeze your glutes while driving the bar up overhead. Complete the movement with your arms in the lockout position overhead. There is only one dip in the push press, and that is when you push the bar overhead. There should not be a second dip at the top of the bar path or that movement would be called a “jerk.” MUSCLES USED: Glutes, quads, hamstrings, shoulders and core.
Finally, although performing the lower trapezius strengthening exercise as described by Kuhn (standing with the arms at the sides and moving the shoulders into extension against resistance of an elastic band) is appropriate for individuals with moderate to high pain levels or altered scapulothoracic movement patterns, other exercises have demonstrated5 greater electromyographic activity levels of the lower portion of the trapezius muscle. The prone “Y” exercise (arm raised in line with the fibers of the lower trapezius) produces high levels of lower trapezius electromyographic activity and might be more effective for strengthening this muscle.5 After an individual's pain resolves and scapulothoracic movement patterns normalize, an athletic trainer or physical therapist might progress the individual to a more challenging position, such as the prone “Y” exercise.

If sit-ups give you a sore neck, try this alternative. Lie flat with the end of a resistance band or towel tucked under the center of your back. Bend your knees and grab the other end of the band above your head. Inhale and use your ab muscles to slowly peel your body up, letting your head rest against the band. Exhale and return to the starting position. Do five reps, making sure your abs do all the work.
The baseline characteristics of the study participants are presented in Table 1. No differences between the training groups existed at study entry. In both groups, men spent more hours in sedentary behavior and had significantly higher weight, height, and VO2peak compared to women. Contrary, more women than men performed at least 30 min of daily physical activity (Table 1). The included participants had higher VO2peak (11%) compared to those with no exercise logs. They also had higher VO2peak (17%) and height (1.7%) compared to dropouts, but a lower BMI (3.7%) (p < 0.05). A higher proportion of the included participants performed 30 min of daily physical activity compared to the dropouts (77.3% vs. 66.1%, p < 0.05).
Many exercise videos will make unrealistic guarantees in terms of the results you can expect to see. Beware of these because they can set you up for a real disappointment. A good example of this is a program that claims you can get “ripped” in 30 days. Well, this might be true IF you are only toning up and don’t have weight to lose. For anyone who has got pounds to lose, they finish the 30 days and are still not “ripped” because those claims did not apply to anyone who has weight to lose.
This reformer exercise is an efficient way to work the entire lower body.  Kneel on the reformer and round the back, keeping the arms straight. Use the butt muscles and thighs to push and pull your lower body back and forth. The platform will slide a few inches with each movement. Do five reps. As you get more advanced, do another five reps with the back arched.
Ready to take it to the next level? This workout with Chloe Bent is a full-length, 30-minute calorie burner that’s filled with dance moves that hit all the major muscle groups. After this dance routine, you’ll feel like your living room just became a stage. Take on this bodyweight routine at home when you need to spice up your cardio regimen. If you’re a beginner, don’t fret: This will be a great challenge for you.

Stability moves train your core to stay strong and steady. They also target your transverse abdominis, the deep ab muscle that does a 360 around your waist and draws it in, says Brent Brookbush, president of the Brookbush Institute of Human Movement Science in New York City. Try this stabilizer: Hold a dumbbell in your right hand and balance on your left leg. Squat on your left leg as you lower the weight toward your left foot. (More moves to try: grab a Bosu ball and challenge your core with this stability workout. Or you can snag one of these balance boards to work on your core as you watch your favorite TV show.)
A compound exercise is a move that incorporates multiple muscle groups, like lunges, deadlifts, and squats. It may also refer to two moves being strung together, like a bicep curl to a shoulder press. Compound exercises are efficient for increasing overall muscle mass and burning calories (because they require more effort to complete), as opposed to isolation exercises, which focus on working just one muscle group (like a bicep curl).
An essential move to any workout. Keep in mind that if doing a push-up on your toes is too tough, you can always start on your knees. It’s still a very effective strengthening move. HOW TO DO IT: Begin the push-up in a plank position with your hands on the ground under your shoulders and with your feet together, toes driving into the ground. Your body should be in one straight line with your core locked. Slowly lower yourself down to the ground so that your chest touches the ground, then push yourself back up to the starting position without collapsing your lower back. MUSCLES USED: Shoulders, triceps, biceps and core.
In Week 1 you’ll perform three sets of every exercise per workout, which over the course of the week adds up to nine sets total for each bodypart, a good starting volume for your purposes. With the exception of crunches for abs, you’ll do 8–12 reps per set. This rep scheme is widely considered ideal for achieving gains in muscle size (the scientific term is hypertrophy) and is commonly employed by amateur and pro bodybuilders alike.
The deadlift is one of the foundational strength movements in any exercise program. HOW TO DO IT: The deadlift begins with the bar on the ground. You can perform this with a regular grip or an alternating grip, which means one hand facing towards your body and one hand facing away. With a proud chest and locked core, pull the bar up while keeping it as close to your body as possible. Use your hip hinge and push your knees back to keep your body over the bar. Then extend the hips and squeeze the glutes to complete the move. MUSCLES USED: Glutes, quads, hamstrings, calves, back and core.
If your immune system is constantly fighting both its internal and external environment, it can be more challenging to live, work and exercise with the greater cognitive dysfunction, headaches, allergies, tender lymph nodes and both joint and muscle pain.  Yes, I do get it. However, I want you to be successful. Living myself with M.E, and lyme co-infections, the right approach is essential to making exercise work for us, not against us. 
Frequency, intensity, type, location and social setting (alone vs. together with others) of exercise were assessed using exercise logs from 618 older adults (aged 70–77 years) randomized to MCT or HIIT. All participants completed exercise logs after each exercise session they performed during one year. Pearson Chi-square tests were run to assess the association between intensity, type, location and social setting of exercise with training group.
“Foam rolling is a form of massage (or trigger point release) that you can do to loosen tight muscles to help improve your mobility,” says Lefkowith. Using a foam roller helps smooth out “knots” in your fascia (the layer of connective tissue surrounding your muscles), which can get in the way of your range of motion. This is crucial for performing exercises with correct form and making sure the right muscle fibers are firing away. While you can stop, drop, and foam roll anytime, it’s often recommended to spend a few minutes with the foam roller before your workout to help get the juices flowing.
Here’s how Chickedantz says to do it: Lay on a flat surface with your knees bent. Place one hand on your upper chest and the other on your belly, just below your rib cage. Breathe in slowly through your nose so that you can feel your stomach move out against your hand. There should be little to no movement in the hand resting on your chest. Open your mouth slightly and slowly exhale completely until you feel your stomach fall and ribcage depress. Pause for two counts and inhale again.

"Look for something with a lot of short segments," says Neporent. "This way, you can do a 10-minute set and you're not committed to a longer routine." When you're ready, you can add the segments together and the workout will still flow. DVD technology has made it easier for video exercisers to do short segments or put several together, depending on their ability
One of the most recognized MMB pioneers was Joseph Hubertus Pilates, born near Dusseldorf in Germany to a prize-winning gymnastics father and naturopath mother. According to Pilates historians’, he was a bullied child who suffered asthma and a weak body that he overcame as a teenager by learning anatomy from medical books and the practice of sports such as wrestling and gymnastics. During this process, he developed a model body, which was even displayed in anatomy classes.39,40 Balanced Body, Inc. [Internet]. Origins of Pilates. 2015 Aug 30 [cited 2015 Aug 30]. Available from: https://www.pilates.com/BBAPP/V/pilates/origins-of-pilates.html.
Recruiting lasted 6 months starting from September 2013. Participants were recruited by means of family doctors to whom the goal of the study was explained. The recruitment flow chart is shown in Figure 1. Three hundred and fifty people aged ≥ 65 were invited to participate. Of these, 51.4% agreed to be included in the screening list while 48.6% refused to participate, mainly for family reasons such as illness/hospitalization/old age of a family member. Forty people were found eligible to participate in the research protocol. Randomly, twenty were assigned to VE and twenty to the control group. The latter were instructed not to take part in any physical activity throughout the study period. All the selected participants signed an informed consent. The study was performed according to the Declaration of Helsinki and approved by the local ethics committee on September 23, 2013.
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