Results of the present study show that ACTH and PRL responses to a double maximal exercise bout are sensitive for the diagnosis of OTS and NFO. Cortisol and GH responses were much less sensitive measures as were resting hormone concentrations. Maximal lactate concentrations at both exercise tests showed a high sensitivity for the detection of OTS, but almost half of the NFO patients did not reach [La]max of 8 mmol l−1 either.

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Recruitment criteria were one or both of sedentariness and dysmetabolism. Thus, we selected subjects who were not physically active or involved in any exercise program; that is, they had a sedentary lifestyle. Moreover, before entering the study, they were carefully screened for metabolic problems which attested a dysmetabolic status, as increased levels of plasma glucose, free fatty acids, triglyceride, and urate in fasting state. Both criteria were verified by means of family doctor databases of subjects.
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