The OLDE protocol and neuromuscular function tests were performed on a Cybex NORM isokinetic dynamometer (CMSi, Computer Sports Medicine Inc., Stoughton, USA). The axis of the dynamometer was aligned with the knee axis, and the lever arm was attached to the shank with a strap. Two shoulder harnesses and a belt across the abdomen limited extraneous movement of the upper body. Full description of the OLDE protocol can be found in Pageaux et al. . Briefly, this protocol allows isolating the knee extensor muscles during a dynamic exercise involving an active isotonic knee extension (from 10 deg to 90 deg, 0 deg = knee fully extended) and a passive knee flexion. The passive flexion angular velocity was set up at 300 deg/s automatically cushioned by the dynamometer for safety purposes. Due to this cushion, the passive knee flexion angular velocity was ~ 180 deg/s. According to a previous study , a cadence of 50 contractions per minute (cpm) was chosen (knee extension angular velocity ~ 106°/s). Subjects maintained a cadence of 50 cpm at all visits via the use of a metronome. Power output produced by the subject was controlled according to the formula:
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1) The biggest critique I have is that transitions from poses are too aggressive and, in many cases, FAR too quick. This could very easily result in stabilizer injury with those healing from core, back, spine or neck injuries or those who don't have the best core strength to begin with. The example that comes to mind is in the 'Sweat" workout. The rapid change from low lunge into a one-foot balanced runner caused an injury for me the first week, and just again today after 6 weeks. If you have ANY history of car crash with spinal involvement, low back problems, abdominal surgery, or core weakness, you MUST listen to your body carefully during these workouts. The modifications are helpful, but they simply decrease the impact of the position once you are in them. The quick transitions in PiYO keep heart rates up, but they also jeopardize the safety of joints or muscles that are a) fatigued from participating and b) unstable due to weakness. Adapt and SLOW DOWN when needed. Better to do 2 sets safely than 4 sets and getting hurt.
Neuromuscular function tests were performed pre and post-exercise to quantify muscle fatigue. As previous studies documented the extent of isometric muscle fatigue induced by OLDE [8, 11, 17, 18], we chose to focus only on isokinetic muscle fatigue. Therefore, knee extensors (KE) MVCs were performed at 60 (MVC60), 100 (MVC100) and 140 (MVC140) deg/s pre (after the warm-up) and post-exercise (13 ± 4s after exhaustion). Subjects were asked to perform two maximal isokinetic knee extensions at each angular velocity (starting position corresponded to knee angle at 90 deg; range of motion was the same as the OLDE). The highest peak torque value of the two trials was considered, and a 20 s recovery was set between each set of KE MVCs. The order of contractions was randomized between sessions as follow (60-100-140 deg/s, 100-140-60 deg/s or 140-60-100 deg/s) and identical for testing pre and post-exercise of the same session. This randomization allows obtaining a time course of KE MVC torque recovery following the time to exhaustion test at each angular velocity was obtained at a different time point at each session: either shortly after exhaustion (13 ± 4 s after exhaustion), 20 s following exhaustion test (P20) and 40 s following exhaustion test (P40). An overview of timing can be found in Fig 1. Twenty seconds after completion of the last KE MVC, a maximal isometric MVC of the knee flexors was performed (isometric KF MVC). Visual feedback of the torque and strong verbal encouragement were provided for each MVC [please see reference 9 for more details].
The only measures that accurately distinguished NFO from OTS were increases in ACTH and PRL concentrations after a second maximal exercise bout. The OTS athletes showed a very small or no increase in ACTH and PRL concentrations after the second exercise bout; the NFO athletes showed very large increases. This is a confirmation of our previous studies with this protocol.10 22 The use of two bouts of maximal exercise to study neuroendocrine variations showed an adapted exercise-induced increase of ACTH, PRL and GH to a twoexercise bout.10
(2) Active Phase (between 60% and 84% HRR). Continuous dynamic and interval training mode exercise involving large muscle activities with an increasing level of difficulty and intensity. Subjects began with a short walk, alternated with various step exercises (e.g., both side and forward-backward step up and down on the platform, with alternate footsteps). Then, they went on performing alternate upper-limb lifts (while keeping inferior limbs flexed) and lower limb flexions and extensions (knee lifts, both side and forward-backward leg lifts, and leg curls), as a sort of brief and easy sequence to be repeated for a fixed time. Integrated multiple plane exercises for upper and lower limbs using elastic resistances (Xertube®) completed the last part of the Active Phase. To reach the goal of gradually augmenting the intensity of the program, the coach continuously checked the HRR level of subjects who were progressively increasing the duration and the number of exercises. The resistance of the elastic bands was also increased by one level (from very light to medium) every 4 weeks.