Go online for more information, recommends certified personal trainer Paula Zurowski. Web sites like collagevideo.com or Zurowski's exercisevideosreviews.com offer detailed descriptions and ratings of fitness videos. Collage even offers a one-minute clip of most videos, so you can get a feel for the level of the workout and whether you're going to like the instructor.
Often, when you watch someone lifting weights in a gym, you’ll notice they’re essentially “throwing” the weights up and “dropping” the weights down more than actually “lifting” or “lowering” the weights. They’re allowing certain aspects of physics (momentum, inertia, and gravity) to do much of the work for them instead of truly engaging, and therefore stimulating their muscles. Unfortunately their “perceived” goal is to make the weight move, but the real goal in weight training isn’t just moving the weight; the goal is to fatigue and challenge the targeted muscles. Depending on the specific exercise and range of movement involved, we instruct our clients to take approximately 10 seconds to lift the weight and another 5-10 seconds to lower the weight. By moving slowly, you’re not allowing inertia to carry the weight up or using gravity to let the weight crash down during the lowering phase of the movement. This enhanced and more complete muscle fiber stimulation ensures that you’re not simply “spinning your wheels.” This high-quality exercise stimulus will lead to greater results far quicker than more traditional lifting methods.
This study was supported by grants from the Liaison Committee for education, research and innovation in Central Norway, The K.G Jebsen Foundation for medical research and the Research Council of Norway. The funding organizations had no role in the design and execution of the study, in the collection, analysis, and interpretation of the data, or in the preparation, review or approval of the submitted manuscript.
Stretching helps maintain flexibility. We often overlook that in youth, when our muscles are healthier. But aging leads to a loss of flexibility in the muscles and tendons. Muscles shorten and don't function properly. That increases the risk for muscle cramps and pain, muscle damage, strains, joint pain, and falling, and it also makes it tough to get through daily activities, such as bending down to tie your shoes.
Indian yoga gurus started arriving to the West at the turn of the 20th century, however, yoga as it is known today only evolved as a popular method to ‘stay healthy and relaxed’ during the second half of the century.50 Syman S. The subtle body: the story of yoga in America. New York (NY): Farrar, Straus & Giroux; 2010. [Google Scholar] There was no contact found between the sporadic early yoga arrivals to the West and the MMB pioneers discussed within this research. However, Eastern mind–body practices profoundly influenced Randell and Morris, most probably via Vaughan, who had just returned from years of obstetric work and eye-opening behavioral observations in India prior to the inception of the St Thomas project in 1912.
For Paced Audio Workouts: MotionTraxx (iPhone or iPad) - This music-based app is another great option for home exercisers, particularly if you're a walker or runner. Deekron the Fitness DJ has put together an incredible variety of music mixes, all set at different beats per minute, so you can find the perfect pace for any workout - Walking, running, lifting weights or other activities. There are also coached workouts (these cost extra) available at iTunes.
Our methodology, classes + 1:1 training incorporate what a lot of fitness methods are missing [regardless of pregnancy]. So while we provide you necessary tools and guide you to a strong + functional core and body, we won’t just teach you to “kegel”  or  “pull your belly button to your spine” because those techniques need to stay in the past. Instead, we create a balance in your pelvic floor and core that will continue to support you for life, preparing you for your birth marathon and motherhood with every pulse, hold, and full range move we guide you through. 
We've said it before, but HIIT really does the job when you want to trim ab fat: A study published in the Journal of Sports Medicine and Physical Fitness found that people who did two HIIT and two strength sessions a week lost more visceral fat (11 percent of the dangerous kind around your organs)—about an extra inch from their waist—than those who ran twice and did two strength sessions. Plus, many of those speedy intervals, such as sprints, are total-body moves that engage your abs big time. Do speed bursts on a cardio machine or try three-minute boxing rounds (another transverse tightener) with a minute of active recovery in between. This unique HIIT workout incorporates some boxing moves and some weight training for double the benefits. (Don't get along with HIIT training? Studies show adding music will make it more enjoyable.)
One almost overall finding, at least in endurance and strengthendurance athletes having OTS, is a diminished maximal lactate concentration, whereas submaximal values remain unchanged or slightly reduced.10 12 This is confirmed in the present study where OTS patients did not reach maximal lactate concentrations above 8 mmol l−1. Two out of the four NFO patients did not reach [La]max of 8 mmol l−1 at the first exercise test either (for one patient [La]max was missing). Thus, although low [La]max has frequently been described as a diagnostic marker for OTS, from these results, it does not seem sensitive enough to distinguish OTS from NFO.
First, we must follow the same guidelines and general protocols for building a stronger ‘foundation’ as we have outlined in the fibromyalgia protocol articles here on this website. The idea is to build a stronger core and immune status. After we have created a support system for the immune and nervous system involvement, we can begin to incorporate an exercise program best suited for fibromyalgia chronic fatigue syndrome.
Regular aerobic exercise improves symptoms associated with a variety of central nervous system disorders and may be used as an adjunct therapy for these disorders. There is clear evidence of exercise treatment efficacy for major depressive disorder and attention deficit hyperactivity disorder.[43][48][52][53][54][55] The American Academy of Neurology's clinical practice guideline for mild cognitive impairment indicates that clinicians should recommend regular exercise (two times per week) to individuals who have been diagnosed with this condition.[56] Reviews of clinical evidence also support the use of exercise as an adjunct therapy for certain neurodegenerative disorders, particularly Alzheimer’s disease and Parkinson's disease.[57][58][59][60][61][62] Regular exercise is also associated with a lower risk of developing neurodegenerative disorders.[60][63] A large body of preclinical evidence and emerging clinical evidence supports the use of exercise therapy for treating and preventing the development of drug addictions.[64][65][66][67][68] Regular exercise has also been proposed as an adjunct therapy for brain cancers.[69]

The effects of exercise training appear to be heterogeneous across non-mammalian species. As examples, exercise training of salmon showed minor improvements of endurance,[155] and a forced swimming regimen of yellowtail amberjack and rainbow trout accelerated their growth rates and altered muscle morphology favorable for sustained swimming.[156][157] Crocodiles, alligators, and ducks showed elevated aerobic capacity following exercise training.[158][159][160] No effect of endurance training was found in most studies of lizards,[158][161] although one study did report a training effect.[162] In lizards, sprint training had no effect on maximal exercise capacity,[162] and muscular damage from over-training occurred following weeks of forced treadmill exercise.[161]
The most important thing you need is you. Just grab a yoga mat, water, and towel and find a space wide enough to take a step in each direction. The PiYo 60-Day Calendar comes with the program and strategically lays out your workout schedule to keep you progressing each week as you build your strength, flexibility, and stamina. The Get Lean Eating Plan gives you a simplified approach to clean and lean eating designed to fit your lifestyle.
"The saying in CrossFit is that we don't use machines, we are the machines," adds Amy "Pistol" Mandelbaum, owner and head coach of CrossFit Westport. "We use barbells, dumbbells, rowers, kettlebells, and 'rigs' for pull-ups. A CrossFit box is like Tinkertoys for adults. Everything is mobile and can be configured to accommodate different movements. Many exercises are bodyweight-oriented, such as burpees, push-ups, jump rope, pull-ups, running, and more."
HIIT stands for high-intensity interval training. “This refers to tough quick, intense bursts of exercise, followed by short recovery periods. This type of training gets and keeps your heart rate up,” explains Laferrara, while also (typically) decreasing the overall amount of time you spend training. This workout is great for burning fat because the intense intervals help kick-start the process known as excess post-exercise oxygen consumption (AKA the “afterburn effect”), which helps you burn more calories even after you stop working out as your body has to work harder and take in more oxygen to return to its resting state.
Just shy of an hour long, this video is a killer aerobic kickboxing workout. You’ll throw punches and kicks in supercharged sequences as you follow along with the ebullient Billy Blanks. Don’t be surprised if you start talking back to the screen, especially when Blanks looks straight into the camera and declares, “I see you at home! Keep going!” Talk about motivation.

The wall sit, also known as a static squat, is performed by placing one's back against a wall with feet shoulder width apart, and lowering the hips until the knees and hips are both at right angles. The position is held as long as possible. The exercise is used to strengthen the quadriceps. Contrary to previous advice in this section, this exercise is NOT good for people with knee problems because the knees bear most of the load, especially when they are held at right angles (90 degrees).[citation needed]
Our method is an extremely important addition to any childbirth class or current fitness method that you love being a part of. We believe that by adding our methodology to your pregnancy, we can keep you doing what you love in the safest and most effective way possible. This method was developed with all stages of pregnancy in mind and our clients continue to see endless benefits from incorporating The Bloom Method into their regular fitness routine, for life.
We’ve gone on the record with our love of MMA conditioning exercises, and that’s why we bookmarked this video. Even the warm-up is jam-packed with explosive movements that’ll get your heart pumping (think high knees and walking front kicks). And once you move into the actual workout, you’re in for even more high-energy exercises, like hopping front kicks, that are sure to condition your body from head to toe.
The European Commission - DG EAC - Directorate General for Education and Culture - has dedicated programs and funds for HEPA - Health Enhancing Physical Activity projects[134] within its Horizon 2020 and Erasmus+ program, as research showed that too many Europeans are not physically active enough. Financing is available for increased collaboration between players active in this field across the EU and around the world, the promotion of HEPA in the EU and its partner countries and the European Sports Week. The DG EAC regularly publishes a Eurobarometer on sport and physical activity.
Aerobic exercise induces mitochondrial biogenesis and an increased capacity for oxidative phosphorylation in the mitochondria of skeletal muscle, which is one mechanism by which aerobic exercise enhances submaximal endurance performance.[98] [92][99] These effects occur via an exercise-induced increase in the intracellular AMP:ATP ratio, thereby triggering the activation of AMP-activated protein kinase (AMPK) which subsequently phosphorylates peroxisome proliferator-activated receptor gamma coactivator-1α (PGC-1α), the master regulator of mitochondrial biogenesis.[92][99][100]
In both groups, men had a significantly higher proportion of sessions at a gym compared to women (Fig. 6). Contrary, women had a higher proportion of sessions at a sports facility compared to men. In the MCT group, men had a significantly higher proportion of sessions outdoors compared to women, while the opposite was observed in the HIIT group (Fig. 6).
Notice in the workouts below that your first set calls for eight reps, your second set 10 reps and your third set 12. This is referred to in bodybuilding circles as a “reverse pyramid” (a standard pyramid goes from higher to lower reps), where you decrease the weight each set to complete the higher rep count. For example, if on your first set of lat pulldowns you used 140 pounds for eight reps, try using 120 or 130 pounds on set two and 100–120 pounds on set three.
In addition to determining the optimal position from which to initiate an exercise based on the patient's related impairments and level of pain, exercise dosage and progression are important aspects of a rehabilitation program. Intervention details, such as number of repetitions and sets, exercise order, and work-to-rest ratios, should be tailored to each patient based on his or her specific needs. The proposed protocol does not describe a method to determine the initial exercise intensity or the criteria for modification or progression. Using a criterion-based method to determine the initial intensity and progression would individualize these guidelines of the exercise program. In their randomized controlled trial designed to address the effectiveness of exercises to treat RCIS, Lombardi et al6 used a 6-repetition maximum load to establish the starting intensity of strengthening exercises. They6 also recommended a reevaluation every 2 weeks to make necessary adjustments to exercise intensity. Although we do not know whether the 6-repetition maximum-load criteria used in their study is optimal, it is an excellent example of a criterion-based method to determine initial exercise intensity and progression. Future research on exercise for the treatment of RCIS should include criterion-based methods to determine the optimal exercise dosage and progression.
Do you even lift, bro? While putting away groceries, do bicep curls with cans, bottles, or other objects. You can also try holding these objects above your head for ten seconds before putting them away. Alternately, when grocery shopping, opt for a basket instead of a cart when you can. You'll be working out your upper body without even thinking about it.
We prefer 1-min incremental exercise testing on a cycle ergometer rather than constant work studies because of its speed, repeatability, and ease of identification of the anaerobic threshold. Although values such as VO2 and anaerobic threshold from both types of studies are reported to be comparable, we questioned whether VD/VT and AaPO2, which depend on simultaneous arterial blood and mixed ... [Show full abstract]Read more
Choose clothes that suit your activity. In general, wear clothes that won’t restrict your movement or blood flow. For some forms of exercise, like biking, you might want to wear form-fitting clothes, but they still shouldn’t be too tight. Looser workout clothes are better for strength training, brisk walking, and sports such as basketball or soccer.[35]
Add tai chi to your exercise routine. Tai chi is a Chinese martial art that involves sequences of slow movements. It can help improve your balance, flexibility, and concentration, and it’s a good way to manage stress. Since it’s a low impact form of exercise, it’s a good option if you’re elderly, have a history of medical issues, or have recently suffered an injury.[28]

While the focus of Pilates is strength training, you'll get some cardio in with moves like this. Stand with your belly pulled in and your arms overhead. Inhale and lower your head, bending the knees and swinging the arms back. Exhale and jump up with straight legs, reaching the arms overhead. Land with the knees slightly bent and return quickly to starting position. Do 8-10 reps at a rapid pace. You should be out of breath when you finish.

At the twilight of his career, despite his personal success and loyal group of followers, Pilates was disappointed his philosophy was not adopted by all. However, a group of his followers became recognized mind–body educators in their own right. His dream was realized by them in the 1980s, when Contrology moved into and has since remained within mainstream acceptance and popularity as ‘Pilates.’39,42 Balanced Body, Inc. [Internet]. Origins of Pilates. 2015 Aug 30 [cited 2015 Aug 30]. Available from: https://www.pilates.com/BBAPP/V/pilates/origins-of-pilates.html.
An evidence-based journal club of 9 faculty members and fellows reviewed the articles and extracted and tabulated the data. Individual outcomes for pain, range of motion (ROM), strength, and function were organized. Intragroup and between-groups outcomes were assessed for the effectiveness of treatment, and statistical outcomes were recorded when available. Clinical importance was determined when statistical value was P < .05 and the effect size or difference between treatments was 20% or more. Sixa major categories were created to organize the components of the physical therapy programs used in each study: ROM, flexibility and stretching, strengthening techniques, therapist-driven manual therapy, modalities, and schedule. Components from these categories were used to create a synthesized physical therapy program.
We’ve gone on the record with our love of MMA conditioning exercises, and that’s why we bookmarked this video. Even the warm-up is jam-packed with explosive movements that’ll get your heart pumping (think high knees and walking front kicks). And once you move into the actual workout, you’re in for even more high-energy exercises, like hopping front kicks, that are sure to condition your body from head to toe.
Mix-and-match interval training works magic in Natalie Jill's Rev4 Rev It Up. The four 10-minute routines hit different trouble zones so you can do them as stand-alones—"I definitely felt I got a good workout after each," one tester said—or combine them for a total-body session. Testers loved that they could "switch things up for time-pressed mornings" and gave props to instructor Jill's "nice energy." Expect a variety of planks and booty-shaping moves.
Also important to know is how to determine how much weight you should use. Start with a light weight and perform a set. Continue adding weight until you can do the desired number of reps with good form, which includes moving slowly enough that you're using muscle—and not momentum—to lift the weight. The last rep should be difficult, but not impossible, and you should be able to keep good form while doing it.

Exercise videos are probably one of the most purchased items when it comes to fitness. They are also one of the most likely to end up on the shelves of users due to many different reasons. In many cases, it’s because the user didn’t know what they were getting into when they purchased the exercise videos, so once they got them home and watched them, it turned out it wasn’t what they were looking for at all.


The good-morning is a weight training exercise in which a barbell, two dumbbells, or no weight at all is held on the shoulders, behind the head. The person bends forward and bows at the hips and recovers to upright. The good-morning is so called because the movement resembles bowing to greet someone. It involves the hamstrings but is primarily used to strengthen the lower back; the degree of knee bend used will change the focus – nearly straight-legged involving the hamstrings most.
Multiple component community-wide campaigns are frequently used in an attempt to increase a population's level of physical activity. A 2015 Cochrane review, however, did not find evidence supporting a benefit.[122] The quality of the underlying evidence was also poor.[122] However, there is some evidence that school-based interventions can increase activity levels and fitness in children.[15] Another Cochrane review found some evidence that certain types of exercise programmes, such as those involving gait, balance, co-ordination and functional tasks, can improve balance in older adults.[123] Following progressive resistance training, older adults also respond with improved physical function.[124] Survey of brief interventions promoting physical activity found that they are cost-effective, although there are variations between studies.[125]

Whether you're allergic to the gym, want to save money or crave convenience, exercising at home is an easy choice to make. What's more difficult is figuring out what to do. How do you set up an effective home workout? What do you do if you don't have much equipment or space? The following series takes you through a variety of choices for exercising at home, whether you have nothing but your own body and a pair of shoes or a workout room tricked out with every piece of equipment imaginable.


One new exercise is added to each bodypart routine to provide even more angles from which to train your target muscles to promote complete development. You’ll hit each muscle group with two exercises of 3­–4 sets each: four sets for large bodyparts (chest, back, shoulders, quads, hamstrings) and three sets for smaller bodyparts (biceps, triceps, abs, calves). The result is 16 total sets for the week for large bodyparts and 12 sets total for smaller ones—again, working in the 8–15-rep range—which is a substantial increase in volume from Week 1.
Personal trainer James Shapiro has a tough yet effective way to get your triceps toned and defined with “body weight skull crushers.” He says to “start in a pushup position either on the floor or on an incline. Have your hands inside shoulder width and fingers point straight ahead of you. Focusing on only bending from your elbows—which should remain tucked into your sides and not flared out—go down feeling the stretch and focus on your triceps.”

The Bloom Method is a unique system that is based on solid evidence compiled by our founder through her years of working individually with thousands of women in pregnancy, early post birth and well into motherhood. The Bloom Method combines cutting edge-core techniques, breathing practices, functional [mom] movements, strength training, Lagree [pilates] based moves, HiiT [for postnatal moms] and groundbreaking philosophies into one life-changing exercise method. 
Jump up ^ Möhlenkamp S, Lehmann N, Breuckmann F, Bröcker-Preuss M, Nassenstein K, Halle M, Budde T, Mann K, Barkhausen J, Heusch G, Jöckel KH, Erbel R (200). "Running: the risk of coronary events : Prevalence and prognostic relevance of coronary atherosclerosis in marathon runners". Eur. Heart J. 29 (15): 1903–10. doi:10.1093/eurheartj/ehn163. PMID 18426850.
Fit septuagenarians may even need to be held back: “Strength training is super empowering,” she says. “And people get excited when they see and feel the results. I have older clients doing multiple timed sets of kettlebell swings. One older client biked 2,700 miles in 50 days. It takes a little longer, but they can reach really impressive levels of fitness.”

Tabata Intervals. The great thing about many of these techinques is the time saving aspect, and Tabata Intervals are definitely time savers. Developed by Dr. Izumi Tabata for Olympic athletes, Tabata Protocol is a form of High Intensity Interval Training (HIIT) where 20 seconds of work is coupled with 10 seconds of rest then repeated for 8 total rounds Metabolic profile of high intensity intermittent exercises. Tabata, I., Irisawa, K., Kouzaki, M., et al. Department of Physiology and Biomechanics, National Institute of Fitness and Sports, Kanoya City, Japan. Medicine & Science in Sports & Exercise 1997 Mar;29(3):390-5.. The 20 seconds work/10 seconds rest pattern has been shown to tax both aerobic and anaerobic pathways more — and in less time — than intense exercise with longer rest periods, meaning improved overall cardiovascular fitness. This protocol can be done with running/rowing/swimming, bodyweight exercises, or weighted movements.
Weighing yourself and keeping an exercise journal are two ways to track your progress, but taking your measurements (chest, arms, waist, hips) will give you a little more information. For example, you may be losing inches even if your scale weight doesn't change. In that case, monitoring your measurements every few weeks can reassure you that you are, in fact, slimming down.
Wildman S. Kafka's Calisthenics. Slate [Internet]. 2011 Jan 21 [cited 2015 Aug 30]. Available from: http://www.slate.com/articles/life/fitness/2011/01/kafkas_calisthenics.html.  He was conferred a knighthood by the King of Denmark in 1919 and his work was granted patronage by the Prince of Wales in 1925.15,16 Müller JP. My system. London: Link House; 1904.
Park further away. I know a lot of us have developed the (bad) habit of trying to get the parking spot absolutely closest to our destination—I know this because I have both cut-off and been cut-off by some aggressive drivers who really wanted to park 50 feet closer—but this is dumb. Spare yourself the road rage and do your body a favor by parking further away and getting in some extra walking.
'Time exposed' to training, in both the intervention and control arms of the study, was defined as the length of time an individual spent in training with his or her original training group free of AKP. Patients were thus censored at the point they were removed from training (various time-points through the 14-week training period). Participants who successfully completed training with their original troop were censored at the point of exit (14 weeks). There was no follow-up after the 14-week point.
I have used the standard protocol (10 seconds plus or minus two for both concentric and eccentric contractions) of this method, although sometimes I prefer going a bit faster such as 4/4, 6/6 or 8/8 seconds respectively. It is not easy and even a bit painful to do a single set of each exercise and "inroad" the muscles. Moving from machine to machine between exercises with no rest, one experiences tremendous cardio-respiratory workload (experiences counterpulsation due to very large venous return to the heart) and some feel extreme fatigue when finished. A workout can last as little as 10-12 minutes, based on 5 basic compound exercises (ie: ankle raise, trunk extension, squat, shoulder or chest press, pulldowns), and personally, I found I needed 2 workouts/week in order to achieve the strength gains I desired. Some think only one session per week is needed and I believe this is a personal and time management choice.

Checkley, Müller, Alexander, and Pilates initiated their interests from a self-requirement to improve health or overcome functional loss. They subsequently used their bodies as a model to demonstrate their method’s effectiveness and encourage others. Despite their turn of the 20th century separation from the gymnasium ‘Physical Culture’ and new independence, all six MMB pioneers advocated their exercises as an adjunct to other sports and regular daily activity; Müller recommended running on the balls of the feet as an aerobic activity, Pilates worked with dancers, while Morris, who besides her dedication to dance and dancers’ health also published ‘Tennis by Simple Exercises’ in 1937 together with French tennis mega-star Suzanne Lenglen.60 Lenglen S, Morris M. Tennis by simple exercises. London: Heinemann; 1937. [Google Scholar]
Tabata training is a form of high-intensity interval training. It consists specifically of eight rounds of high-intensity exercise using a 20 seconds on (at full capacity) and 10 seconds off (rest) format. The goal is to push yourself as hard as you can during the 20 second on period. Tabata only requires one exercise, but you can combine different strength and aerobic exercises to make your own Tabata routine. Here’s a simple example of Tabata for one exercise:
Yoga also offers many mental benefits, such as a reduction in stress level. Greater relaxation and reduced stress leads to a host of other positive body changes including an improvement in circulation, sleep and self-confidence. A positive mental outlook also helps to maintain a healthy immune system, which in turn can ward off other serious ailments such as high cholesterol, heart disease and stroke.
You won’t find another person like Brooke, in this whole world. The inspiration and motivation she carries is way beyond what most people hold. the knowledge she has about pregnancy and post pregnancy exercise is something you will want to connect with, and continue to work with. Taking a step to work with Brooke is making a choice to support your whole life: family, baby, body, mind, spirit. thank you Brooke for making exercise and fitness such an essential and safe part of pregnancy.
After trying many different workout "schemes" with limited success, I bought this book and began doing Super Slow workouts. I'm now working out 6 times a month, spending less than 30 minutes in the gym for each workout, and I'm stronger than I've ever been. I've never experienced progress like this before. At 45 years old, my leg press has gone from 400 to 820 lbs. in a couple of months. The workouts aren't easy, but they're over quickly, and I'm able to spend more time with my family without feeling like I'm compromisng my health and fitness.

I love this DVD because it's basically the equivalent of seven DVDs in one — offering seven different 20-minute yoga practices to mix and match. I liked the freedom of being able to do a different one each day or combine two when I wanted to do a longer session. The flows range from slow-paced for relaxation to more intense for an energizing practice.
In total, 1567 participants (790 women) met the inclusion criteria, fulfilled baseline testing and were randomized 1:1 into an exercise training group or to a control group. The exercise training group was further randomized 1:1 to either MCT or HIIT. Participants in the exercise groups were instructed to fill in exercise logs after each exercise session they performed. Data in the present study is based on the exercise logs from the first year of the intervention. Therefore, only participants in the exercise groups were included in the present study (n = 787). Dropouts in the exercise groups during the first year (n = 123) and those with no exercise logs (n = 46) were excluded. A total of 618 participants (291 women) were included in the analyses (Fig. 1). The study was approved by the Regional Committee for Medical Research Ethics (REK sør-øst B: 2015/945) and all participants gave their written informed consent before participation.
Samples were collected in prefrozen 4.5 ml K3 EDTA vacutainer tubes (Becton Dickinson Vacutainer System Europe, Plymouth, UK) and immediately centrifuged at 3000 rpm (Minifuge 2, Heraeus, Germany) for 10 min, and plasma was frozen at −20°C until further analysis. Samples were assayed via RIA for cortisol (DiaSorin, Stillwater, Minnesota, USA), ACTH (Nichols Institute Diagnostics, San Juan Capistrano, California, USA), PRL (Roche Diagnostics, Mannheim, Germany) and GH (Pharmacia & Upjohn Diagnostics, Uppsala, Sweden).
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